Literature summary extracted from

Jung, J.; Braun, J.; Czabany, T.; Nidetzky, B.
Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli (2019), Catal. Sci. Technol., 9, 842-853 .

No PubMed abstract available

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.7.1.13	expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.7.1.13	C190A	the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity	Escherichia coli
1.7.1.13	C190S	the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity	Escherichia coli
1.7.1.13	D197A	the variant does not take up protons in conjunction with preQ0 binding	Escherichia coli
1.7.1.13	D197H	the variant recovers proton uptake to a level almost analogous to that seen with the wildtype enzyme	Escherichia coli
1.7.1.13	H229A	the variant readily forms the thioimidate adduct and is 24fold less active for preQ0 reduction than wild type enzyme	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.7.1.13	7-cyano-7-deazaguanine + 2 NADPH + 2 H+	Escherichia coli	i.e. preQ0	7-aminomethyl-7-deazaguanine + 2 NADP+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.7.1.13	Escherichia coli	Q46920	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.7.1.13	immobilized metal ion affinity column chromatography and gel filtration	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.7.1.13	7-cyano-7-deazaguanine + 2 NADPH + 2 H+	i.e. preQ0	Escherichia coli	7-aminomethyl-7-deazaguanine + 2 NADP+	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.7.1.13	QueF	-	Escherichia coli

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Release 2023.1 (January 2023)